Cross 8 - Page Under Construction

Purpose: Fourth backcross to restore the Snow White phenotype

Male (Gandalf):

  • Bought from a big box store during February 2023 as part of the initial stock.

  • Strain: Snow White

  • Relevant genes. Some are assumptions, indicated with italics. Although their decedents strongly support the claims:

    • Base body color: Blond (bb)

    • Magenta: likely homozygous (MM)

    • European Blau: (rr)

    • Stoerzbach: (ss)

Female (C2AF):

  • Gandalf’s daughters from Cross 2.

  • Strain: Half Snow-White

  • Relevant genes. Some are assumptions, indicated with italics. Although their decedents strongly support the claims:

    • Base body color: Gray (Bb)

    • Magenta: likely a carrier (Mm)

    • European Blau: (Rr)

    • Stoerzbach: (Ss)

Offspring:

  • Born:

  • Brood Size:

    • Male

    • Female

  • Offspring phenotypes and genotypes:

    • The figure above shows all the male phenotypes ordered by the relevant genes that showed an obvious phenotype. Base body color, European Blau, and stoerzbach: I will use the grid as my grouping labels.

    • The females followed a similar phenotype distribution except for stoerzbach. I had a hard time telling apart females that expressed stoerzbach.

    • Genotype distributions. Numbers indicate the distribution with-in just the males excluding the juveniles.

      • A1: Bb|(MM or Mm)|Rr|Ss

      • B1: Bb|(MM or Mm)|Rr|ss

      • C1: Bb|(MM or Mm)|rr|Ss

      • D1: Bb|(MM or Mm)|rr|ss

      • A2: bb|(MM or Mm)|Rr|Ss

      • B2: bb|(MM or Mm)|Rr|ss

      • C2: bb|(MM or Mm)|rr|Ss

      • D2: bb|(MM or Mm)|rr|ss

    • My expectation was that each of the 8 unique phenotypic combinations (resulting from three genes) would represent roughly 12.5% (indicated by the dotted line) of the offspring. This is because the C1B female is heterozygous for each gene, while Gandalf is homozygous (similar to her C1A sister minus the halfblack gene). This means each individual gene should show an even 50% distribution of its two phenotypes. At first glance, the overall phenotypic distribution seemed skewed towards B1, and C2. However, after examining the distributions of each gene individually, the picture becomes much clearer:

      • Base Body Bolor:

      • European Blau (EB):

      • Stoerzbach:

Notes:

  • A YouTube video detailing the start of Cross 8 can be found here.